Osmolyte perturbation reveals conformational equilibria in spin-labeled proteins
نویسندگان
چکیده
منابع مشابه
High-pressure EPR reveals conformational equilibria and volumetric properties of spin-labeled proteins.
Identifying equilibrium conformational exchange and characterizing conformational substates is essential for elucidating mechanisms of function in proteins. Site-directed spin labeling has previously been employed to detect conformational changes triggered by some event, but verifying conformational exchange at equilibrium is more challenging. Conformational exchange (microsecond-millisecond) i...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2009
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.180